clinimacs prodigy automated t cell transduction tct process (Miltenyi Biotec)

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Miltenyi Biotec clinimacs prodigy automated t cell transduction tct process
Clinimacs Prodigy Automated T Cell Transduction Tct Process, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cells/CliniMACS+Prodigy/nct05779930-0-53-52
Average 98 stars, based on 798 article reviews

clinimacs prodigy automated t cell transduction tct process - by Bioz Stars, 2026-09

98/100 stars

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Single Cell:

Article Title: Kinematic‐calcium loops unravel impaired excitation–contraction coupling in MELAS‐affected cardioids
Article Snippet: Between 21 and 26 days after the differentiation started, the CMs were purified from the non-CMs fraction through an immunomagnetic-aided purification kit (catalog. no. 130-110-188; Miltenyi Biotec, Bergisch Gladbach, Germany). .. In summary, the cells were detached and resuspended in a single-cell suspension with an enzyme-based protocol (catalog. no. 130-110-204; Miltenyi Biotec). ..

Suspension:

Flow Cytometry:

Article Title: Stable, long-acting mesenchymal stromal cell secretome delivery via injectable starPEG-heparin hydrogels for sustained immunomodulation.
Article Snippet: The secretome of mesenchymal stromal cells (MSCs) has emerged as a promising cell-free therapeutic strategy for regenerative medicine and immune modulation.. However, its clinical translation remains limited by restricted stability and the short in vivo half-life of its bioactive factors, often requiring repeated administrations.. Here, we describe an injectable star–shaped poly(ethylene glycol)–heparin (starPEG–heparin) hydrogel that enables the stable loading and sustained release (up to 14 days) of a lyophilized immunomodulatory MSC secretome (LS).

Labeling:

Article Title: Regulation of dendritic cells maturation via the Serpinb8/ITGAX axis in H. pylori-induced gastric mucosal injury.
Article Snippet: Background: Helicobacter pylori (H. pylori) has evolved multiple immune evasion mechanisms that enable its persistent colonization in the gastric mucosa of approximately half of the global population.. The chronic inflammation resulting from this persistent infection is widely recognized as a major contributor to gastric carcinogenesis.. The specific goal of this study is to elucidate how H. pylori infection regulates dendritic cell (DC) maturation via the Serpinb8/ITGAX axis.

Magnetic Cell Separation:

Staining:

Article Title: CSF1R regulates monocyte subset differentiation and intracellular metabolism.
Article Snippet: .. For intracellular staining, cells were fixed and permeabilized using Miltenyi Foxp3 staining buffer (cat #130-093-142). ..

Blocking Assay:

Article Title: Jagged1 + Tregs mediate lymphatic remodeling in tumor-draining lymph nodes.
Article Snippet: Human tumor and sentinel LN tissue flow cytometry Liquid nitrogen frozen human sample vials were thawed and rested in 10mL of 10% FBS RPMI 1640 media containing 1MU/mL of DNase I (Sigma-Aldrich; 260913-25MU) for 1 h in 37◦C 5% CO2 incubator. .. Upon resting, 2 million cells per sample were added with FcR blocking reagent (Miltenyi Biotec; 130-059-901) diluted in FACS buffer (5% FBS in PBS) for 20 min at 4◦C then washed with FACS and centrifuged at 300 g for 5 min. ..

FACS:

Incubation:

Article Title: Identification and enrichment of human retinal organoid-derived red/green cone-competent precursors with enhanced axon dynamics.
Article Snippet: Magnetic-activated cell sorting (MACS) using anti-CD166 Singularized cells from L/M-CRR ROs at D50 were incubated with Biotin-conjugated ALCAM/CD166 (Miltenyi Biotec 130-126-100; 1:50 dilution) for 20 min at 4°C and washed three times with MACS Separation buffer (autoMACS Rinsing Solution (Miltenyi Biotec 130- 091-222) containing 0.5% MACS BSA Stock Solution (Miltenyi Biotec 130-091-376)). .. Thereafter, cells were incubated with Anti-Biotin ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS microbeads (Miltenyi Biotec 130-090-485; 1:10 dilution) and passed through an LS column (Miltenyi Biotec 130-042-401) placed on a magnetic stand. ..

Review